Culture of normal human airway epithelial cells and measurement of mucin synthesis and secretion.

Sildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro

P-113: Survey of In Vitro Effect of Resveratrol on Nitric Oxide Secretion of Human Endometrial Epithelial Cells

Background: Resveratrol is a natural polyphe- nolic compound, synthesized by plants as a phytoalexin and protects against ultraviolet radiation and fungal infection. Nitric oxide is formed from L-arginine through nitric oxide synthases (NOS), a group of enzymes that structurally resemble cytochrome P-450 reductase. In females, circulating NO is increased during follicle development and decrease...

Stimulation of mucin secretion from human bronchial epithelial cells by mast cell chymase.

AIM To investigate the effect of chymase on the mucin secretion from human bronchial epithelial cells. METHODS Primarily-cultured human bronchial epithelial (PCHBE) cells and normal human bronchial epithelial (NHBE) cells were cultured with chymase or other stimulus in a mixture of bronchial epithelial growth medium (BEGM) and Dulbecco's modified Eagle's medium (DMEM), and the quantities of s...

Airway epithelium and mucus: intracellular signaling pathways for gene expression and secretion.

It is the rare scientific paper dealing with any aspect of airway mucus that does not open with a statement about the contribution of excess mucus to the pathogenesis of airway obstruction, susceptibility to infection, or compromised defense in a myriad of inflammatory airway diseases, such as chronic bronchitis, asthma, bronchiectasis, or cystic fibrosis. Excess mucus in the airways can result...

Mir-21 Regulation of MARCKS Protein and Mucin Secretion in Airway Epithelial Cells

Hypersecretion of mucus characterizes many inflammatory airway diseases, including asthma, chronic bronchitis, and cystic fibrosis. Excess mucus causes airway obstruction, reduces pulmonary function, and can lead to increased morbidity and mortality. MicroRNAs are small non-coding pieces of RNA which regulate other genes by binding to a complementary sequence in the target mRNA. The microRNA mi...